Figure 4.

A deficiency of RGS13 in BXD2 mice obstructs age-related increase of GCs but enhances the numbers of IgM plasma cells in young mice. A, FACS analysis of the percent of PNA⁺Fas⁺ CD19⁺ GC B cells in the spleens of 1.5- and 6-mo-old mice (mean ± SEM of the percent of gated population within B cells, N=4; ** p<0.01, *** p<0.005 for the indicated strain in comparison with BXD2-WT mice). B, FACS analysis of the percent of CD138⁺ (Syndecan-1) B220⁺ plasmablasts in B6, BXD2, and BXD2-Rgs13^−/− mice, at 1.5-mo-old. C, D, Left, Low power (10× objective lens) and Middle, zoomed view: Representative photomicrographs of immunohistochemical staining of IgM^bright and IgG^bright cells in the spleens of (C) 1.5-mo-old and (D) 6-mo-old mice. Right, Quantitation of the number of IgM^bright and IgG^bright cells per 10× objective lens microscopic view (mean ± SEM. N=4 mice per group). At least 3 randomly chosen areas were counted per section (** p<0.01 or *** p<0.005 between the indicated comparisons, for Panels B - D).