Figure 6.

A deficiency of RGS13 in BXD2 mice limits AID expression and antibody affinity maturation. A, Confocal immunofluorescent assessment of position of PNA⁺ or AID⁺ B cells and CD4 T cells in the GCs. Yellow rectangle gated areas were enlarged to reveal the contact between CD4 T cells (white) and PNA⁺ GC B cells (red) or between CD4 T cells (white) and AID⁺ B cells (green). Bar graph showing the average numbers of PNA⁺ GC B cells or AID⁺ GC B cells surrounding each CD4 T cell in the GCs. At least 3 GCs were analyzed per section with 4 sections per strain (** p<0.01, as compared to BXD2-WT mice). B, C, ELISA analysis of the serum (B) high affinity anti-NP and (C) high+low affinity anti-NP IgG₁ and IgG_2b antibody responses at the indicated days after immunization of mice with NP₂₁-CGG (N=4 mice per group; * p<0.05; ** p<0.01; *** p<0.005 as compared to BXD2-WT mice). All Data are shown as mean ± SEM.