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. 2014 Apr 8;9(4):e94198. doi: 10.1371/journal.pone.0094198

Figure 1. Estrogen biosynthesis and antioxidant activities of the crude samples from the BPL.

Figure 1

(A) KGN cells were seeded in 24-well plates and pretreated with the crude samples at 50 μg/mL for 24 h. The cells were then supplemented with 10 nM testosterone for further 24 h, and 17β-estradiol in the culture medium was quantified using a magnetic particle-based 17β-estradiol ELISA. (B) ABTS and DPPH solutions were prepared daily and diluted to an absorbance of 0.70±0.02 at 734 nm (ABTS) and 517 nm (DPPH). After the crude samples (10 μg/mL) reacted with the ABTS radical solution for 10 min (DPPH radical solution for 30 min), the absorbance value (Ai) of ABTS at 734 nm (or Ai of DPPH at 517 nm) was measured, and the percentage inhibition was calculated. Cont., DMSO control; FSK, 10 μM Forskolin; Let, 1 μM letrozole; Vc, ascorbic acid; GT, methanol extract; GC, chloroform soluble extract; GB, n-butanol soluble extract; GW, aqueous extract.