Figure 5. The estrogen biosynthesis activity of the isolated compounds from the BPL.

KGN cells were seeded in 24-well plates and pretreated with test chemicals at 50 μM for 24 h. The cells were then supplemented with 10 nM testosterone for further 24 h, and 17β-estradiol in the culture medium was quantified using a magnetic particle-based 17β-estradiol ELISA. Cont., DMSO control; FSK, 10 μM Forskolin; Let, 1 μM letrozole.