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. 2014 Apr 8;9(4):e94187. doi: 10.1371/journal.pone.0094187

Figure 2. Muscle proteins were significantly enriched among the differentially expressed (DE) genes.

Figure 2

(A) The significantly enriched GO categories (y-axis) in the DE genes with FDR <0.05. (B) The Log2 fold changes from both RNA-seq (dark) and arrays (grey) of the DE genes associated with muscle proteins and contractile fiber, cytoskeleton protein binding and heart development. (C) The ZBED6 peak at about 1 kb upstream of Twist2 gene (grey box) displayed together with placental mammal conservation score. The peak maxima overlapped with a highly conserved region (grey box), which contained the consensus motif GCTCGC of ZBED6 only in the placental mammals. A 10-bp insertion (+10) was present within the consensus motif in the opossum genome, indicating lack of ZBED6 binding site in this region. (D) The relative expression levels of Twist2 from RNA-seq and array in either Zbed6-silenced or control C2C12 cells. (E) The relative luciferase activity (the ratio of firefly to Renilla) of Igf2, Twist2 and empty pGL3 basic constructs in Zbed6-silenced or control C2C12 cells. **, P<0.01; ***, P<0.001.