Skip to main content
. 2014 Apr 8;9(4):e94187. doi: 10.1371/journal.pone.0094187

Figure 5. ZBED6 modulates the expression of Igf2 and Myogenin during differentiation of C2C12 cells.

Figure 5

(A) Expression of ZBED6, IGF2 and Myogenin mRNA monitored by qPCR before and after differentiation of control cells and transfected cells overexpressing ZBED6 from the pTRE-ZB vector. (B) Expression of ZBED6, IGF2 and Myogenin mRNA monitored by qPCR before and after differentiation of control cells and cells in which ZBED6 has been silenced using siRNA. (C) Western blot analysis confirming altered expression of ZBED6 and Myogenin at the protein level. Actin was used as loading control.