Table 3. Overrepresentation analysis of transcription factor binding sites within 2- or downstream of the transcription start site of genes showing significant differential expression after Zbed6-silencing in C2C12 myoblast cells.
| Transcription | TF | Up-/Down- regulation | Enrichment | Expression analysis | ||
| factor | class | na | Pb | Mc | Pd | |
| NFKB1 | REL | down | 125 | 1.54E-04 | −2.03 | 0.01 |
| ELK4 | ETS | down | 180 | 3.99E-04 | −1.15 | 0.01 |
| SP1 | C2H2 | down | 318 | 3.24E-02 | −0.21 | 0.54 |
| MEF2A | MADS | up | 98 | 1.27E-10 | −0.58 | 0.22 |
| PRXX2 | HOMEO | up | 186 | 4.72E-08 | 0.52 | 0.23 |
a
Number of genes containing the transcription factor binding site.
b
Fisher's exact test.
c
M-values represent log2 transformation of fold changes between Zbed6-silenced samples and negative controls estimated by qPCR analysis. Triplicates for each treatment were used.
d
Student's t-test were used to calculate the statistical significance.