Figure 1. Memo KO fibroblasts have defects in PDGF-induced cell migration.

A, Diagram for conditional Memo deletion in mouse embryonic fibroblasts (MEFs). Immortalized Memo fl/fl MEFs were infected with a CreERT²-expressing retrovirus then treated with tamoxifen (TAM). B, Western analyses for Memo levels in lysates from cells subjected to the indicated treatments; α-tubulin is the loading control. CreERT²-expressing Memo fl/fl MEFs without or with TAM treatment were used as control and Memo knock-out (KO) MEFs, respectively. C, Transwell cell migration of control and Memo KO MEFs was induced by PDGF (5 ng/ml) in serum-free media. Data were normalized to the average value for basal migration in serum-free media and are presented as means ± S.D. of five individual wells. Similar results were obtained in three independent experiments. **, p<0.01; ***, p<0.001