Table 2.
Results of the orthogonal experiment
| (A) Inner water phase volume (mL) | (B) VLPVPR (mg) | (C) Outer water phase volume (mL) | (D) Ultrasound of double emulsion | DL (%) | ER (%) | |
|---|---|---|---|---|---|---|
| 1# | 0.1 | 5 | 1 | 5 s ×1 | 2.96 | 60.97 |
| 2# | 0.1 | 7.5 | 2 | 5 s ×2 | 3.67 | 50.86 |
| 3# | 0.1 | 10 | 3 | 5 s ×3 | 2.37 | 24.25 |
| 4# | 0.2 | 5 | 3 | 5 s ×2 | 2.82 | 57.99 |
| 5# | 0.2 | 7.5 | 1 | 5 s ×3 | 6.31 | 89.8 |
| 6# | 0.2 | 10 | 2 | 5 s ×1 | 5.06 | 53.34 |
| 7# | 0.4 | 5 | 2 | 5 s ×3 | 2.28 | 46.65 |
| 8# | 0.4 | 7.5 | 3 | 5 s ×1 | 2.37 | 32.38 |
| 9# | 0.4 | 10 | 1 | 5 s ×2 | 5.07 | 53.44 |
| DL | ||||||
| K1 | 9.00 | 8.06 | 10.39 | 14.34 | Calibration parameter =120.38 | |
| K2 | 14.19 | 12.36 | 11.57 | 11.02 | ||
| K3 | 9.72 | 12.50 | 10.96 | 7.56 | ||
| S | 5.27 | 4.26 | 0.23 | 7.67 | ||
| F | 23.02* | 18.58 | 1.00 | 33.51* | ||
| ER | ||||||
| K1 | 136.08 | 165.61 | 146.69 | 204.21 | Calibration parameter =24,511.03 | |
| K2 | 201.13 | 173.04 | 162.29 | 150.85 | ||
| K3 | 132.47 | 131.03 | 160.70 | 114.62 | ||
| S | 995.41 | 335.09 | 49.13 | 1,354.03 | ||
| F | 20.26* | 6.82 | 1.00 | 27.56* | ||
Notes:
*
Significant (P<0.05) by Student’s t-test.
Abbreviations: DL, drug loading; ER, encapsulated ratio; F, statistical analysis results for orthogonal experiment; K, the average DL and ER of nanoparticles under the various investigated conditions; S, standard deviation; s, seconds; VLPVPR, Val-Leu-Pro-Val-Pro-Arg.