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. 2014 Apr 8;9(4):e94302. doi: 10.1371/journal.pone.0094302

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© 2014 Zhang et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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A. Ethanol extracts prepared from 500 Chinese herbs. B. Natural compounds fraction library constructed by preparative HPLC with a linear gradient of 0–90% methanol. Eighty fractions were collected from the ethanol extract of each herb, which line up with hydrophobicity gradient. C. Determination of a randomly selected fraction by analytical HPLC. D. Working library. Each fraction was dissolved in DMSO to make 5 mg/mL working solution. Each 96-well plate contains 80 fractions from one herb.