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. 2014 Apr 8;9(4):e94302. doi: 10.1371/journal.pone.0094302

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© 2014 Zhang et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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A. A representative plate with positive fractions in screening by cell-based fluorescent assay. Data from the 96-well plate of Chinese wild grapevine showing 14-s time courses (I⁻ added at 2 s) of EYFP-H148Q fluorescence. Controls (C: saline; N: cocktail; P: cocktail plus 20 μM CFTR_inh-172) are shown on the right. Asterisks indicate positive wells with decreased I⁻ influx (representing CFTR inhibition). B. Percentage inhibition rate of the positive fractions indicating Gaussian distributions of the positive clusters. C. Activity of positive fractions. Dose-response results showing inhibition of cocktail-stimulated (5 μM forskolin, 100 μM IBMX and 25 μM genistein) I⁻ influx by fraction 51 (left) and fraction 55 (right) at indicated concentrations. Mean ± SE is shown from three sets of experiments.