Figure 6. Synergistic enhancement of anti-angiogenesis effect of lidamycin with TMZ in subcutaneous U87 tumor sections. The microvessle density (MVD) was determined by CD31 immunofluorostain assay, and the images were photographed [(A), 200×] and enumerated (B). Red, CD31-positive blood vessels stained with Cy3-labeled secondary antibody; blue, the cell nuclei counter-stained with 4,6-diamidino-2-phenylindole (DAPI). The VEGF secretion related to angiogenesis were detected by VEGF immunofluorostain in glioma section [(C), 200×). Red, VEGF-positive areas stained with Cy3-labled secondary antibody; blue, the cell nuclei counter-stained with 4,6-diamidino-2-phenylindole (DAPI). The photos were enumerated and plotted (D). Each column represented the mean ± s.d. ***P < 0.001 between indicated groups. In all the photos, the scale bar = 200 μm.