Figure 2. The effects of GATA3 knockdown on bladder cancer cell migration. A wound healing assay was used to assess cell migration of 5637, TCC-SUP, and J82 with or without GATA3-shRNA (A), UMUC3 with or without transfection of a GATA3 plasmid (B), or SVHUC with or without GATA3-shRNA (C). Cells were scratched and cultured with 6 μg/ml puromycin for 24 h. The migration was determined by the rate of cells filling the wound area, and the normalized cell-free areas (24 h/0 h; the ratio in each control line set as one-fold) were quantified. Each value represents the mean + standard deviation from three independent experiments. *P < 0.05. **P < 0.01.