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. 2014 Jan 21;15(4):428–435. doi: 10.4161/cbt.27631

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graphic file with name cbt-15-428-g5.jpg — Figure 5. The effects of GATA3 knockdown on cell proliferation in bladder cancer lines. (A) 5637-parental/GATA3-shRNA/control-shRNA and J82-parental/GATA3-shRNA/control-shRNA cells were cultured for 1–4 d, and cell viability was assayed with MTT. Growth induction is presented relative to cell number at day 1 in each cell line. Each value represents the mean + standard deviation from at least three independent experiments. (B) 5637-parental/GATA3-shRNA/control-shRNA and J82-parental/GATA3-shRNA/control-shRNA lines were analyzed on western blotting, using an antibody to cyclin D1 (36 kDa), cyclin D3 (31 kDa), p21 (21 kDa), p27 (27 kDa), CDK4 (30 kDa), CDK7 (40 kDa), or caspase-3 (35 kDa). GAPDH (37 kDa) served as an internal control. UMUC3-control/GATA3 (C) and SVHUC-GATA3-shRNA/control-shRNA (D) were cultured for 1–4 d, and cell viability was assayed with MTT. Growth induction is presented relative to cell number at day 1 in each cell line. Each value represents the mean + standard deviation from at least three independent experiments.