Figure 6. acALY18 abrogates fibrosis. Cells were cultured until confluent. At Day 0 media was removed and saved for hydroxyproline assay. acALY18 (3 ng/ml) was added to fresh media and the cells cultured for 3 or 4 d. At each addition of fresh media, the expired media was saved for hydroxyproline assessment and fresh acALY18 added. The study was terminated at Day 17. (Left) Hydroxyproline levels in all samples were determined at the same time. (Right) Cells were harvested on Day 17, lysed, and protein analyzed by western blotting for α-smooth muscle actin, collagen 3A1, and β-actin.