Figure 7. Naclynamide dose response curve. (Top) Dose response curve obtained with primary human fibroblasts cultured for 48 h in the presence of increasing concentrations of Naclynamide. Secreted IL-1β was measured at the 48 h time point by ELISA. Each data point is the mean of 3 separate experiments. Dotted lines represent the regression curve and the calculated EC₅₀ and EC₉₉ from a semi-log plot (middle) and a Hill Plot (bottom) of the data.