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. Author manuscript; available in PMC: 2015 Jan 22.
Published in final edited form as: J Am Chem Soc. 2013 Oct 23;136(3):826–829. doi: 10.1021/ja408814g

Figure 2.

Figure 2

Determination of post-amplification DNA labeling efficiency via streptavidin (SA) gel shift. (A) Labeling strategy (illustrated for dTPT3PA-dNaM; B = biotin). (B) Labeling with unnatural base pair centrally located in 134mer duplex. The faster and slower migrating bands corresponds to dsDNA, and the 1:1 complex between dsDNA and streptavidin, respectively. (C) Labeling with unnatural base pair positioned at first, ninth, or eleventh positions, corresponding to duplexes 1–3, respectively.