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. Author manuscript; available in PMC: 2015 Jan 22.

Published in final edited form as: J Am Chem Soc. 2013 Oct 23;136(3):826–829. doi: 10.1021/ja408814g

Table 1.

Kinetic and PCR amplification data.

	Presteady-state kinetics		OneTaq PCR (48 cycles)			Taq PCR (20 cycles)
	Presteady-state kinetics
dXTP	%inc^a	%ext^b	amplification ×10¹²	retention, %	fidelity, %	amplification ×10³	retention, %	fidelity, %
5SICS	57.0 ± 0.2	15.1 ± 1.1	9.4	96.3 ± 1.7	99.91 ± 0.04	7.7	86.7 ± 1.0	98.90 ± 0.01
FPT1	7.2 ± 0.2	32.0 ± 1.5	9.7	62 ± 2	98.89 ± 0.09	10.9	42.0 ± 1.1	93.73 ± 0.18
TPT1	28.7 ± 0.5	8.8 ± 0.2	13.3	60 ± 7	98.8 ± 0.3	6.7	33.76 ± 0.03	91.81 ± 0.01
TPT2	65.7 ± 0.5	34.5 ± 0.5	16.6	96.8 ± 0.9	99.90 ± 0.02	9.9	93.05 ± 0.04	99.46 ± 0.01
TPT3	72.3 ± 0.5	49.8 ± 1.3	12.9	> 99	>99.98	11.7	95.6 ± 1.7	99.66 ± 0.13
FTPT3	66.3 ± 0.5	33.8 ± 0.2	6.5	97.9 ± 1.0	99.95 ± 0.02	5.0	98 ± 3	99.9 ± 0.2
TPT3^PA	68.3 ± 0.4	31.5 ± 0.7	4.7	98.6 ± 1.2	99.97 ± 0.03	3.5	85 ± 4	98.7 ± 0.4
5SICS^PA	7.0 ± 0.2	5.5 ± 0.1	9.2	45 ± 2	98.16 ± 0.12	6.4	-^c	-^c

^a

Incorporation assay conditions: 40 nM unnatural triphosphate, 2 μM dCTP, 10 s.

^b

Extension assay conditions: 10 μM unnatural triphsophate, 2 mM dCTP, 10 s.

^c

Unnatural base pair was lost during amplification.