Table 2. In vitro (18 hrs) As+3, As+5 or MMA+3 Exposure and Effects on Mouse Bone marrow Cell Viability1 .
Treatments | % Viability (Cellometer) | % Annexin V negative/PI negative cells | % Annexin V positive/PI negative cells | % Annexin V negative/PI positive cells |
Control | 63.2+1.4 | 78.1+2.7 | 16.1+2.5 | 5.7+0.2 |
5 nM As+3 | 63.3+0.6 | 77.8+2.2 | 16.3+2.1 | 5.8+0.8 |
50 nM As+3 | 58.4+3.5 | 76.2+1.3 | 17.8+0.6 | 5.8+0.5 |
500 nM As+3 | 59.8+1.7 | 75.6+2.0 | 18.4+0.4 | 6.0+1.7 |
5 nM As+5 | 59.3+0.1 | 77.8+1.8 | 16.7+0.3 | 5.4+1.4 |
50 nM As+5 | 58.8+3.7 | 75.9+1.4 | 17.1+1.5 | 6.7+0.4 |
500 nM As+5 | 62.7+2.2 | 74.3+0.8 | 19.2+1.2 | 6.4+0.5 |
5 nM MMA+3 | 59.3+3.5 | 76.5+1.9 | 17.6+2.0 | 5.8+0.2 |
50 nM MMA+3 | 59.9+6.8 | 78.2+0.5 | 16.6+0.5 | 5.0+0.6 |
500 nM MMA+3 | 39.5+10.0* | 52.0+2.5* | 21.6+3.4* | 26.4+2.2* |
Bone marrow cells from three mice femurs were pooled and exposed to each treatment in triplicate. Results are Means + SD with statistical significance at *p≤0.05.