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. 2014 Apr 8;9(4):e94713. doi: 10.1371/journal.pone.0094713

Figure 3. Whole mount analysis revealed enlarged collecting lymphatic vessels of the hind limb.

Figure 3

CD31 (A, green) and αSMA (B, red) stainings of collecting lymphatic vessels from chow diet fed mice were compared to CD31 (D) and αSMA (E) stainings of collecting lymphatic vessels from HFD fed mice. The collecting lymphatic vessels were observed to be enlarged in the HFD fed mice. CD31 (G, green) and αSMA (H, red) stainings of non-contractile ear collecting lymphatic vessels from chow diet fed mice were compared to CD31 (J, green) and αSMA (K, red) stainings from HFD fed mice. No major differences were observed. Merged channels are shown in (C, F, I and L). Scale bars  =  50 μm.