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. 2014 Apr 8;9(4):e93974. doi: 10.1371/journal.pone.0093974

Figure 5. CA inhibits serum withdrawal-induced outgrowth of axon-like cell processes and protection by Ber in N2a cells.

Figure 5

The cells were cultured in serum-free (SF) medium for 12 or 24 h, then 2.5 nmol/L CA was added to the 12 h SF group and these cells were cultured for another 12 h. The morphology of the cells was photographed by a phase contrast microscope (A). The mean length of the cell processes (B, excluding those with zero length of the processes) and the proportion of cells with different lengths of the processes (C) were calculated by a stereological system. About 100–200 axon-like cell processes for each treatment were counted. Data were mean ± SD (n = 5). ##p<0.01 versus SF 12 h, **p<0.01 versus SF 24 h, &&p<0.01 versus SF 12 h+ CA 12 h. Bar  = 10 μm.