Figure 2. Purification of biofilm-degrading activity for peptide mass fingerprinting. (A) The fractions obtained by chromatography of the L. gummosus extract on a ProPac-SAX10 column were tested for biofilm-degrading activity (ST, starting material; FT, flow-through; W, wash; C, water control). (B) The fractions were analyzed by SDS-PAGE followed by staining with Flamingo Fluorescent Gel Stain. The bands labeled 1, 2, and 3 were excised and digested in-gel with trypsin for peptide mass fingerprinting. Fractions 8 and 17 were digested in solution with trypsin for peptide mass fingerprinting.