Figure 3. Phylogeny of L. gummosus enzymes that co-purified with biofilm-degrading activity. The phylogenetic trees were constructed on the basis of the amino acid sequences of the predicted preproenzymes using Geneious Tree Builder software (Blosum62; gap open penalty, 3; gap extension penalty 3). Alyt, Achromobacter lyticus; Lenz, L. enzymogenes; Lgum, L. gummosus; Lsp, Lysobacter sp.; Paer, Pseudomonas aeruginosa. The proteins in the fractions with biofilm-degrading activity are marked with an asterisk. Accession numbers are listed in the supplemental material.