Figure 1. CENP-A expression declines in the adult heart. (A) qPCR analysis of CENP-A transcription (n = 3). (B) Immunoblot of CENP-A protein expression and quantification (n = 3). (C) Representative staining of CENP-A (green), tropomyosin or actin (red), and the nuclear stain topro (blue) in the pre-natal heart (left panel) and 3-mo-old heart (right panel). (D) Representative staining of c-kit (red) positive stem cells expressing CENP-A (green) and pH3 (white) in the pre-natal heart with respective magnified images. pH3 expression is detected in the mitotic stem cell shown in the far right panel but is absent from the non-mitotic stem cell in the center panel. The nuclear stain sytox is represented in blue. (E) Representative staining of c-kit (red) positive stem cells expressing CENP-A (green) together with actin (white) and nuclei (blue) in the 3-mo-old heart. **P < 0.005, ***P < 0.0005.