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Figure 2. CENP-A declines in old CPCs. (A) Immunocytochemical analysis of CENP-A (green), the actin stain ph633 (red), and nuclear stain sytox (blue) in CPCs cultured in vitro. (B) Immunocytochemical analysis confirming CENP-A staining (green) co-localizes with c-kit (red). (C) Immunoblot demonstrating that CENP-A is significantly diminished in CPCs derived from old mice. Quantification shown in right panel (n = 3). *P < 0.05.
