Figure 4. CENP-A knockdown results in premature senescence in CPCs. (A) Representative bright field images from 2 independent experiments show increased number of β-gal-positive CPCs following CENP-A knockdown (top right panels). Quantification of cell counts reveals 3-fold increase in the percentage of senescent CPCs (n = 4). (B) Immunoblot showing no increase in expression of αSMA in CENP-A KD CPCs relative to ScrCon CPCs. CPCs treated with Dex for 7 d to induce cellular commitment serves as a positive control. Two different exposures of the immunoblot are shown (n = 3). *P < 0.05; NS, not significant. Scale bars = 100 μm.