Figure 5. Complete ablation of CENP-A in Dex-treated CPCs increases apoptosis. (A) FACS analysis of Annexin V staining shows no increase in basal apoptosis in CENP-A KD CPCs (n = 3). (B) Annexin V staining significantly increases in CENP-A KD CPCs following Dex induced differentiation. Representative scatter plots shown in left panels, quantifications in right panels (n = 3). (C) Immunoblot analysis indicating that CENP-A expression declines in CPCs treated with Dex, and is completely absent in CENP-A KD CPCs treated with Dex. Two different exposures of the immunoblot are shown (n = 3). *P < 0.05, NS, not significant.