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. 2014 Jan 6;13(5):749–761. doi: 10.4161/cc.27676

Figure 4.

Figure 4.

Expression of SMARCA2 is modulated by histone deacetylation in SCC-11 cells upon cisplatin exposure. SCC-11 cells and SCC-11M cells were transfected with the scrambled (Scr) miRNA for 32 h, and then exposed to control medium (Con) or 10 μg/ml cisplatin (CIS) for an additional 16 h. Cells were also transfected with the miR-92b-3p mimic, or HDAC9 siRNA and exposed to control media for 48 h. Cells were also treated with the 5 μM MC1568 for 16 h. (A) QPCR assay for the SMARCA2 expression was performed from 3 independent experiments in triplicate (P < 0.05). (B) SCC-11 cells and SCC-11M cells were additionally transfected with 100 ng of the LightSwitch_Pro reporter plasmid for the SMARCA2 promoter for 24 h. Renilla luciferase reporter activity assay was conducted from 3 independent experiments in triplicate (P < 0.05). (C) ChIP-qPCR assay of the HDAC9 binding to the specific region of the SMARCA2 promoter. QPCR assays were performed using 3 independent experiments in triplicate (P < 0.05). The amount of ChIP-enriched DNA (ChIP/Input) represented as a signal relative to the total amount of chromatin DNA (input) using the same primers.