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. 2014 Jan 6;13(5):762–771. doi: 10.4161/cc.27677

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Copyright © 2014 Landes Bioscience

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graphic file with name cc-13-762-g3.jpg — Figure 3. Effects of PluriSin#1 on Nanog-positive iPSD. (A1 and A2) Mouse iPSD were incubated with DMSO or PluriSin#1 for 4 d; note the appearance of cell death in the central region of iPSD treated with PluriSin#1; (B1 and B2) DMSO- and PluriSin#1-treated iPSD were assayed for apoptosis using TUNEL staining; note extensive TUNEL positivity in the center of PluriSin#1-treated iPSD; (C) Real-time RT-PCR analysis demonstrating the effect of PluriSin#1 on Nanog mRNA expression; (D) Protein was isolated from DMSO or PluriSin#1 treated IPSD and then used for immunoblotting; (E1 and E2) Immunostaining for Nanog demonstrating elimination of Nanog-positive iPSD cells by PluriSin#1. Nuclei were counterstained with DAPI; (F and G) Mouse iPSD were incubated with DMSO or PluriSin#1 for 1 d, a combined TUNEL and Nanog immunofluorescent staining demonstrating apoptosis of Nanog-positive iPSD cells by PluriSin#1. Nuclei were counterstained with DAPI.