Figure 3. Exclusive requirement of TC-NER for the removal of transcription-blocking dG(N²)-AAF.

Host cell reactivation in human cell lines of the specified nucleotide excision repair complementation groups (CS-A, CS-B, XP-C and XP-A) and in the repair proficient cell line (MRC-5). The expression constructs contained a unique dG(N ²)-AAF or dG in the specified DNA strand. (A) Overlaid fluorescence distribution plots obtained in a representative experiment. All cell lines were transfected in parallel with the same constructs. (B) Mean relative expression (dG(N ²)-AAF/dG) calculated for multiple transfections with independent preparations of the expression constructs (n = 3, +/− s.d.).