Table 4. The SOD and CAT activities and the GSH and MDA levels in renal, liver, heart, spleen and lung tissues of rats with contrast-induced nephropathy (n=7).
| Tissue / Parameters | Experimental groups |
P | |||
|---|---|---|---|---|---|
| Control | CM | Carnitine | CM + Carnitine | ||
| Renal | |||||
| SOD, U/mg protein | 2.90 ± 0.21a,b) | 2.30 ± 0.20b) | 3.53 ± 0.46a) | 3.16 ± 0.12a,b) | * |
| CAT, k/mg protein | 0.66 ± 0.32 | 0.43 ± 0.20 | 1.03 ± 0.52 | 0.71 ± 0.24 | NS |
| GSH, mg/g protein | 0.38 ± 0.02a) | 0.29 ± 0.02b) | 0.40 ± 0.02a) | 0.38 ± 0.03a) | * |
| MDA, nmol/mg protein | 222.01 ± 19.77c) | 569.54 ± 37.56a) | 351.80 ± 40.18b) | 426.69 ± 52.86b) | *** |
| Liver | |||||
| SOD, U/mg protein | 5.49 ± 0.39a,b) | 3.75 ± 0.34b) | 7.22 ± 0.85a) | 3.93 ± 0.22b) | *** |
| CAT, k/mg protein | 27.30 ± 8.33 | 17.91 ± 3.34 | 28.70 ± 5.59 | 21.66 ± 2.19 | NS |
| GSH, mg/g protein | 1.00 ± 0.14a,b) | 0.38 ± 0.05c) | 1.40 ± 0.22a) | 0.70 ± 0.12b,c) | *** |
| MDA, nmol/mg protein | 165.35 ± 11.36a,b) | 223.92 ± 22.08a) | 109.41 ± 10.67b) | 110.78 ± 8.17b) | *** |
| Heart | |||||
| SOD, U/mg protein | 4.12 ± 0.91 | 2.49 ± 0.54 | 4.39 ± 0.65 | 3.71 ± 0.37 | NS |
| CAT, k/mg protein | 1.16 ± 0.74 | 0.37 ± 0.08 | 1.16 ± 0.74 | 1.03 ± 0.42 | NS |
| GSH, mg/g protein | 0.47 ± 0.08 | 0.29 ± 0.05 | 0.46 ± 0.11 | 0.42 ± 0.10 | NS |
| MDA, nmol/mg protein | 111.04 ± 17.87 | 136.73 ± 38.56 | 90.75 ± 11.42 | 91.11 ± 8.34 | NS |
| Spleen | |||||
| SOD, U/mg protein | 4.07 ± 0.66a) | 2.15 ± 0.43b) | 3.65 ± 0.18a) | 3.27 ± 0.20a,b) | * |
| CAT, k/mg protein | 1.39 ± 0.30a) | 0.42 ± 0.06b) | 1.19 ± 0.14a) | 1.35 ± 0.15a) | ** |
| GSH, mg/g protein | 0.64 ± 0.13b) | 0.27 ± 0.02c) | 1.02 ± 0.10a) | 0.73 ± 0.08b) | *** |
| MDA, nmol/mg protein | 54.07 ± 6.13a) | 61.82 ± 4.06a) | 21.78 ± 1.93b) | 52.90 ± 3.90a) | *** |
| Lung | |||||
| SOD, U/mg protein | 1.31 ± 0.13 | 1.05 ± 0.07 | 1.16 ± 0.06 | 1.12 ± 0.03 | NS |
| CAT, k/mg protein | 0.41 ± 0.11 | 0.32 ± 0.14 | 0.51 ± 0.15 | 0.46 ± 0.09 | NS |
| GSH, mg/g protein | 0.45 ± 0.03a) | 0.30 ± 0.04b) | 0.50 ± 0.03a) | 0.43 ± 0.04a) | ** |
| MDA, nmol/mg protein | 25.79 ± 1.05b,c) | 30.16 ± 0.53a) | 24.13 ± 0.71c) | 27.14 ± 0.95b) | *** |
a, b, c) Different letters indicate statistically significant differences in the same row. * P<0.05; ** P<0.01; *** P<0.001. NS, not significant; CM, contrast medium; CM+carnitine, contrast medium+carnitine treatment; SOD, superoxide dismutase activity; CAT, catalase; GSH, glutathione; MDA, malondialdehyde.