Skip to main content
. Author manuscript; available in PMC: 2015 Mar 21.
Published in final edited form as: Chem Commun (Camb). 2014 Mar 21;50(23):3071–3073. doi: 10.1039/c4cc00432a

Fig. 3.

Fig. 3

Spectral response of BV2+ towards various spiked thiols in reduced human plasma upon irradiation. Absorption spectra of solutions of BV2+ (20 mM) in reduced human blood plasma and 0.5 M Tris buffer (pH 7.0) spiked with 1.5 µM Hcy, 25 µM Cys and 0.6 µM GSH. Plasma was incubated for 1 h with TCEP Gel followed by filtration using a Single StEP™ 0.45 µm PVDF filter vial. The reduced, filtered plasma (10% v/v) was added to an argon-saturated solution of viologen, thiol and buffer and irradiated for 15 min using a Reptisun™ lamp.