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. Author manuscript; available in PMC: 2014 Sep 1.
Published in final edited form as: Stem Cell Res. 2013 May 16;11(2):743–757. doi: 10.1016/j.scr.2013.05.002

Figure 7.

Figure 7

Astrocytes are essential for synaptic maturation of human neurons. (A) Representative images showing SV2-labeled synaptic puncta along the DCX+ neurites of hiPSC-derived neurons developing for two months on laminin or astrocytes. Scale bar = 10 μm. (B) Representative traces showing spontaneous synaptic events recorded from hiPSC-derived neurons after plating hNPCs on laminin or astrocytes for three weeks and two months. (C & D) Quantified results illustrating that hiPSC-derived neurons supported by glial cells consistently show higher frequency (C) and larger amplitude (D) of spontaneous synaptic events than those on laminin. *p < 0.05, **p < 0.01, ***p < 0.001 (student's t-test). (E) Representative traces showing spontaneous synaptic events recorded from human neurons derived from a different iPSC line (WT 33) after plating on astrocytes for six weeks.