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. 2014 Mar 18;10(4):377–385. doi: 10.7150/ijbs.8015

Figure 2.

Figure 2

The CNN3 gene is one of target of miR-1. (A)The 3'-UTR of CNN3 contained the binding site of miR-1. The upper part represents CNN3 and the lower part represents miR-1. The corresponding plot shows conservation across species. (B) miR-1 down-regulated the luciferase activity of the psi-Check2-CNN3-3'UTR construct. The miR-1 mimics and normal control (NC) were transfected into PIECs together with the CNN3-3'UTR vector, the relative luciferase activity was measured 36 h later.(C) The expression of CNN3 is down-regulated by miR-1 at the mRNA level. (D) miR-1 inhibits the translation of endogenous and exogenous CNN3 mRNA. (a) After transfection, the levels of endogenous CNN3 protein decreased, as evidenced by Western blot. (b) miR-1 downregulated the exogenous CNN3 protein, whereas the mutant CNN3 protein exhibited no change compared to the NC. The expression of CNN3 was normalised to GAPDH.