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. Author manuscript; available in PMC: 2015 Apr 15.
Published in final edited form as: J Immunol. 2014 Mar 10;192(8):3548–3558. doi: 10.4049/jimmunol.1302241

Fig 2.

Fig 2

PB, bone marrow, and spleen analyses of WT, PHIL, NJ1638, and NJ1638.PHIL mice. A, The spectrum of naïve- to memory-like phenotype of B cells is characterized by surface expression of CD80, CD273, and CD73. (28, 29) B, PB from WT, PHIL, NJ1638, and NJ1638.PHIL were analyzed for naïve-like/memory-like phenotype as described in (A) or for B1 vs. B2 phenotypes. C, Spleen and bone marrow isolated from a single femur of WT and NJ1638 mice were analyzed for CLP, pre-pro-B, and pro-B cells. Total numbers of B cell progenitors in each mouse were calculated by combining the numbers of spleen and marrow cells using the approximation that the total bone marrow hematopoietic volume is 15.8 times the hematopoietic volume in a single femur (i.e., estimated total hematopoiesis = cells in spleen + (cells in one femur × 15.8) ).(61) * p<0.05; ** p<0.01; *** p<0.001; n.s., not significant.