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. Author manuscript; available in PMC: 2015 Apr 15.

Published in final edited form as: J Immunol. 2014 Mar 10;192(8):3548–3558. doi: 10.4049/jimmunol.1302241

Fig 6 — Activation of Eos is not required for the support of B cell proliferation. A, Eos isolated from human BM and PB and B cells isolated from PB, tonsils, and spleens were analyzed for TLR9 protein expression. Beta actin was used as loading control. B, B cells and Eos were stimulated independently with CpG. Proliferation of unstimulated and CpG-stimulated B cells was assessed either alone or in the presence of unstimulated or CpG-stimulated Eos. C, Eos were cultured alone or with B cells ± CpG or in the presence of various Eos-activating cytokines. Eos were analyzed for surface expression of CD69 on D0, D1, D2, and D3 by flow cytometry (solid grey (), isotype; open black (□), anti-CD69 mAb). Numbers in FACS plots represent △MFI. Data are representative of 3 independent experiments. D-E, B cells or B+Eos cultures were stimulated with various cytokines for Eos activation (D) or B cell activation (E) and proliferation was evaluated. * p<0.05; ** p<0.01; *** p<0.001; n.s., not significant.

Inline graphic — Activation of Eos is not required for the support of B cell proliferation. A, Eos isolated from human BM and PB and B cells isolated from PB, tonsils, and spleens were analyzed for TLR9 protein expression. Beta actin was used as loading control. B, B cells and Eos were stimulated independently with CpG. Proliferation of unstimulated and CpG-stimulated B cells was assessed either alone or in the presence of unstimulated or CpG-stimulated Eos. C, Eos were cultured alone or with B cells ± CpG or in the presence of various Eos-activating cytokines. Eos were analyzed for surface expression of CD69 on D0, D1, D2, and D3 by flow cytometry (solid grey (), isotype; open black (□), anti-CD69 mAb). Numbers in FACS plots represent △MFI. Data are representative of 3 independent experiments. D-E, B cells or B+Eos cultures were stimulated with various cytokines for Eos activation (D) or B cell activation (E) and proliferation was evaluated. * p<0.05; ** p<0.01; *** p<0.001; n.s., not significant.