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. Author manuscript; available in PMC: 2015 Apr 15.
Published in final edited form as: J Immunol. 2014 Mar 12;192(8):3881–3888. doi: 10.4049/jimmunol.1301974

Figure 5. ERK phosphorylation is induced by priming but not by ATP in absence of priming.

Figure 5

ERK phosphorylation was measured in monocyte lysates by immunoblotting with anti-phosphoERK specific antibody and total ERK antibody, respectively. (A) Comparison of ERK phosphorylation induced by LPS (1ug/ml) vs ATP 5mM, respectively. (B) UO126 (10μM) blocks phosphoERK induction by the LPS/ATP combination. (C) MG132 (20μM) blocks LPS induced ERK phosphorylation. (D) ERK phosphorylation in unstimulated versus LPS-primed WT, Nlrp3−/− and Casp1−/−BMDMs.