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. Author manuscript; available in PMC: 2015 Apr 15.
Published in final edited form as: J Immunol. 2014 Mar 12;192(8):3881–3888. doi: 10.4049/jimmunol.1301974

Figure 6. ERK1 knockdown suppresses IL-18 processing and release.

Figure 6

Monocytes were nucleofected with ERK1 and ERK2 specific siRNA or scrambled control and recovered overnight. Cells were then primed with LPS (1μg/ml) for 30 min and activated with ATP (5mM) for another 30 min. Supernatants were analyzed for IL-18 release and cell lysates were immunoblotted to confirm ERK protein down regulation using total ERK antibody. IL-18 ELISA represents the mean± SEM for three separate monocyte donors and the blots are representative of repeated blots. **p < 0.01 as compared to scrambled siRNA.