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. Author manuscript; available in PMC: 2015 Apr 15.
Published in final edited form as: J Immunol. 2014 Mar 12;192(8):3881–3888. doi: 10.4049/jimmunol.1301974

Figure 8. Inhibition of reactive oxygen species generation impairs LPS priming of monocytes and IL-18 release.

Figure 8

Monocytes were pretreated with DPI (1, 10, 20 and 50 μM) for 30 min prior to LPS (1 μg/ml) priming followed by the ATP (5mM) activation step. Supernatants were analyzed for (A) LDH and (B) IL-18 release, respectively. *p < 0.05 and **p < 0.01 as compared to LPS/ATP. (C) Cell lysates were immunoblotted for phosphoERK and total ERK.