Table 1.

Primer sequences, product size and annealing temperature used in this study

Primers	Primer sequences	Product size (bp)	Annealing temperature
RT-PCR
CYB5R2- forward	5′-CCTTGTAGGGACCCGTCCC-3′	291	66 °C
CYB5R2- reverse	5′-GACAGGGGTGTAAGCCCTG-3′
GAPDH-forward	5′-AAGCTCACTGGCATGGCCTT-3′	375	60 °C
GAPDH-reverse	5′-CTCTCTTCCTCTTGTGCTCTTG-3′
Methylation-specific PCR
MSP-forward	5′-GGGGAGCGGGTTAGTCGTC-3′	140	65 °C
MSP-reverse	5′-GAACCCGCAAACTCGTAACGTC-3′
USP-forward	5′-GGGGAGTGGGTTAGTTGTTG-3′	146	58 °C
USP-reverse	5′-CACCACAAACCCACAAACTC-3′
Bisulfite sequencing PCR
BGS-forward	5′-GGTAGGGTTGATTTAGAGTTAG-3′	301	58 °C
BGS-Reverse	5′-CTTCAATACTCCATAAATACACC-3′