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Type IV collagen expression demonstrated by quantum dot-525 (green). a Abundant type IV collagen fragments stochastically distributed in tumor tissues. b Rich type IV collagen in tumour stroma aligning with tumor nests. c, d Different characteristics between HCC (red star) and live cirrhosis (LC) tissues. Red arrowheads show stiff type IV collagen at interface of liver cirrhosis and tumor nests. Red arrows indicate the linear invasion "highways" for tumor cells escape. Scale bar = 50 μm
