Fig. 3.

DNA-PKcs is in close proximity to phospho-Ser473/474-AKT or EGFR in DLD-1 cells. DLD-1 and its corresponding AKT isotype knockout cells were exposed to radiation (1 h post IR 10 Gy) and analyzed with the proximity ligation assay (PLA) of the particular proteins. The interaction between phospho-Thr2609-DNA-PKcs with phospho-Ser473/474-AKT A) as well as between DNA-PKcs and EGFR B) were analyzed before and after irradiation (1 h, post IR10 Gy). The error bars represents the standard deviation from five measurements. The difference was analyzed with Student’s t test where *P < 0.05, **P < 0.01, and ***P < 0.001