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. Author manuscript; available in PMC: 2015 Apr 1.
Published in final edited form as: J Neurochem. 2013 Nov 17;129(2):221–234. doi: 10.1111/jnc.12506

Figure 9.

Figure 9

A, B. Total Internal Reflection Fluorescence (TIRF) microscopy images of paxillin-GFP and mCherry dual-Src homology 2 domain (mCh-dSH2) fluorescent images of a growth cone on PDL-laminin. Note that paxillin and phosphotyrosine (PY), as revealed with mChdSH2, colocalize at adhesion sites (arrowheads in A, B), whereas the tip of a growing filopodium has PY, without paxillin (arrow in B). C. Merged image of the growth cone in (A, B) shows co-localization at several peripheral adhesions. Note that mCh-dSH2 puncta within the central domain are mobile vesicles. D. Single line kymograph constructed from region between the arrowheads in C. Note a stable adhesion (arrow) that disassembles after a new protrusion extends forward, followed by the formation of a second adhesion (arrowhead), which stabilizes the receding protrusion. Scale bar, 10 μm in all images and as indicated in kymographs. With permission from Santiago-Medina et al. (2012) Dev. Neurobiol. 72, 585-599.

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