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. Author manuscript; available in PMC: 2014 Nov 1.
Published in final edited form as: Obesity (Silver Spring). 2013 Dec 4;22(5):1246–1255. doi: 10.1002/oby.20634

Figure 2.

Figure 2

Most CLS macrophages express TNFα while a few express both TNFα and IL-10. Images presented are a LSCM 2D projection of a 3D image stack. Adipose tissue from obese mice that was fixed and stained with BODIPY 558/568 for lipid (gray), Hoechst for nuclei (blue), anti-F4/80 for macrophages (red), anti-TNFα (green), and anti-IL-10 (magenta). The panel shows a four micron 2D projection captured with a 63× objective. The majority of CLS macrophages produce TNFα and some appear to produce both TNFα and IL-10 (arrows) while others have no cytokine expression (F4/80 only).