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Light micrographs of consecutive 2-μm thick sections from kidneys of mouse pups at postnatal day 7, illustrating immunostaining of AQP2, Klotho, and NCC. A) Apical AQP2-positive immunoreactivity identifies the principal cells of the CD (stars). B) Klotho is expressed in the CNT, DCT (asterisks), and CCD (stars). C) Immunoperoxidase labeling of NCC in the DCT (asterisks) of the renal cortex. Scale bars: 20 μm.
