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. 2014 Apr 9;4:4619. doi: 10.1038/srep04619

Figure 3. Comparisons mRNA/protein levels of p63 and Ki67 at different passages (P0 to P3) of the hNESPCs from NP versus healthy controls.

Figure 3

p63 and Ki67 mRNA levels were determined by means of real-time RT PCR (panel A). All target mRNA relative expression levels were given as ratios of PGK1 transcript levels. Line charts are shown with mean ± SE. p63 and Ki67 protein levels were determined by means of immunofluorescence assay (B). Mann-Whitney test was used to analyze the difference of Ki67+/p63+ cell percentage in colonies between NPs and controls at each passage. Symbol “**” means the p-value is less than 0.01, while symbol “***” means the p-value is less than 0.001. Representative pictures show the p63/or Ki67 single-positive cells and p63/Ki67 double-positive cells (panel C). Original magnification 200×. (scale bar = 50 μm).