Effects of P40 anthocyanin extract on cell growth and cell cycle progression in human colon SW480 cancer cells. (A) Treatment of cells with P40 anthocyanin extract at 10–40 μM of peonidin-3-glucoside equivalence for 48 hrs (■) versus the positive peonidin 3-glucoside control (□). Data are means ± SD, n = 3, Means within a treatment without a common letter are significantly different, p < 0.05. (B) Cells co-cultured with P40 anthocyanin extract at 10–40 μM of peonidin-3-glucoside equivalence for 48 hrs were analyzed by flow cytometry as described in the Materials and Methods. Data are means ± SD, n = 3, ** p<0.01 versus the vehicle control.