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. Author manuscript; available in PMC: 2014 Apr 9.
Published in final edited form as: Insect Sci. 2012 Nov 22;20(2):158–166. doi: 10.1111/j.1744-7917.2012.01568.x

Fig. 1.

Fig. 1

The DTT, Mg++, UDP-GlcNAc and GlcNAc dependency of CHS activity in mosquito pupae. (A) Enzyme activity was dependent on DTT in the enzyme extraction buffer. (B) The Mg++ dependent CHS activity. DTT and Mg++ were added to the Tris-HCI buffer for crude enzyme preparation. The concentrations in this figure represented the concentrations in the extraction buffer. UDP-GlcNAc (C) and GlcNAc (D) were added in the reaction mixture and the concentrations in the figure represented the final concentration in the reaction system. The CHS activity values are given in percent of maximal activity and as an average (± SEM) of three independent replicates.