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. Author manuscript; available in PMC: 2014 Oct 1.
Published in final edited form as: Nat Struct Mol Biol. 2014 Mar 9;21(4):405–412. doi: 10.1038/nsmb.2786

Figure 2. Annealing between MH is prevented by RPA.

Figure 2

a, Graph showing survival frequencies and distribution of end joining events in rfa1 hypomorphic mutants. All NHEJ events detected in the rfa1-D228Y, rfa1-t33 and rfa1-t48 mutants were Ade−. b, Western blot of steady-state protein levels of the indicated rfa1 mutants. c, Graph showing survival frequencies and distribution of end joining events in strains over-expressing RFA1 or rfa1-D228Y in the rfa1-D228Y background. d, Viability and genotype of haploid spores derived from diploids heterozygous for sae2Δ and rfa1-D228Y. The genotype of dead spores is inferred by the segregation pattern of sae2Δ and rfa1-D228Y. e, Graph showing distribution of end joined products in the sae2Δ rfa1-t33 mutant. Mean values are shown and error bars represent s.d. from the mean, significance was determined by a 2-tailed Student’s t test. The number of trials for each strain is presented in Table 1.