Table 1.
Frequency of end joining in wild type and mutant strains
| Relevant genotype | Frequency NHEJ1 (x10−4) | Frequency Ade− MMEJ2 (x10−4) | Frequency Ade+ MMEJ (x10−4) | # trials |
|---|---|---|---|---|
| WT | 33.7±10.0 | 1.77 | 0.56±0.25 | 12 |
| yku70Δ | ND3 | 0.59 | 0.79±0.14 | 5 |
| dnl4Δ | ND | 0.63 | 0.45±0.14 | 4 |
| sae2Δ | 265±57.7 | ND | 0.96±0.58 | 6 |
| mre11-H125N | 165±43.0 | 2.86 | 0.87±0.40 | 4 |
| mre11Δ | ND | 0.55 | 3.22±0.95 | 5 |
| exo1Δ | 55.4±11.2 | 1.58 | 1.16±0.45 | 4 |
| sgs1Δ | 35.4±8.86 | 3.21 | 0.68±0.32 | 5 |
| exo1Δ sgs1Δ | 30.7±8.36 | ND | 13.7±3.23 | 4 |
| exo1Δ sgs1Δ yku70Δ | ND | ND | 15.4±3.53 | 3 |
| rad51Δ | 17.1±8.65 | 4.78 | 3.16±0.91 | 4 |
| rad52Δ | 13.1±5.35 | 2.72 | 1.90±0.75 | 4 |
| rad51Δ rad52Δ | 23.9±10.2 | 2.47 | 2.84±0.56 | 4 |
| rfa1-D228Y | 15.6±2.10 | 19.1 | 69.2±17.5 | 5 |
| rfa1-t33 | 23.3±4.07 | 23.3 | 47.5±16.5 | 5 |
| rfa1-t48 | 61.8±32.6 | 41.2 | 196±63.3 | 4 |
| rfa1-t11 | 24.0±6.10 | ND | 0.45±0.26 | 4 |
| rfa1-D228Y rad51Δ | 28.6±11.9 | 11.8 | 137±17.6 | 3 |
| rfa1-D228Y rad52Δ | 21.8±4.75 | 53.3 | 152±18.9 | 3 |
| rfa1-t33 sae2Δ | 137±19.3 | ND | 14.6±2.86 | 6 |
1
Ade− and Ade+ NHEJ events are combined
2
The frequency of Ade− MMEJ could not be accurately assessed in most strains because it represents a minor class of the Ade− survivors as determined by PCR analysis.
3
ND: Not detected.